DMO method for intracellular pH.
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چکیده
منابع مشابه
Intracellular pH of sea urchin eggs measured by the dimethyloxazolidinedione (DMO) method
Intracellular pH (pH1) of sea urchin eggs and embryos was determined using DMO (5,5-dimethyl-2,4-oxazolidinedione). By this method, the pH1 of Lytechinus pictus eggs increased after fertilization from 6.86 to 7.27, and this higher pHi was maintained thereafter, as has been previously observed with pH microelectrodes. The same general result was obtained with the eggs of Strongylocentrotus purpu...
متن کاملThe effect of varying the amount of unlabelled 5,5-dimethyloxazolidine-2,4-dione (DMO) in the measurement of rat hepatic intracellular pH using (14C)DMO.
1. Hepatic intracellular pH (pH,) was measured in intact rats by distribution of 5,5dimethyloxazolidine-2,4-dione (DMO), using [14C]DM0 and a range of doses of unlabelled DMO, previously neutralized with sodium hydroxide. 2. Mean calculated pHi fell from 7-27 to 7-17 as the dose of added unlabelled DMO was increased from zero to 0.19 mmol/kg (25 mg/kg) and thereafter remained constant up to and...
متن کامل(DMO) from the myocardium
1. The efflux of IT-labelled 5,5-dimethyl-2,4oxazolidinedione (DMO) from the myocardium of the rabbit has been studied. The perfusate pH was 7.38. 2. The effluent curve is complex and appears to be the resultant of movement between at least four compartments. 3. The first two probably represent intravascular and extracellular compartments; the last two have smaller rate constants and may repres...
متن کاملRegulation of intracellular pH.
The approach that most animal cells employ to regulate intracellular pH (pH(i)) is not too different conceptually from the way a sophisticated system might regulate the temperature of a house. Just as the heat capacity (C) of a house minimizes sudden temperature (T) shifts caused by acute cold and heat loads, the buffering power (beta) of a cell minimizes sudden pH(i) shifts caused by acute aci...
متن کاملValidation of a Simple and Rapid Method for Assessment of Intracellular Bacterial Asparaginase
L-Asparaginase has remarkable properties which make it useful in dual pharmaceutical and food industries.In this study, simple and advantageous method has been validated for rapid and precise determination of intracellular L-Asparaginasein bacterial species. A suspension of bacterial cells was used instead ofcell extract and incubated by substrate (asparagine) after simple wash and centrifugati...
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ژورنال
عنوان ژورنال: Circulation Research
سال: 1976
ISSN: 0009-7330,1524-4571
DOI: 10.1161/01.res.39.1.141